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• Applied Imaging
• Applied Spectral Imaging, Inc. 

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Applied Imaging
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Applied Imaging competes in the genetic testing and cancer pathology markets. The Company is the leading supplier of automated imaging systems for the analysis of chromosomes in cancer and prenatal disorders. Clinicians and researchers worldwide use our genetic testing systems more often than any other automated system.

The company has expanded its scanning and imaging capabilities, and is adding key reagents, for the detection and genetic characterization of rare cancer cells indicative of metastatic disease. Applied Imaging has placed over 2000 systems in 1000 laboratories in over 40 countries.

PowerGene^™ MacKtype karyotyping system 

PowerGene^™ MacKtype karyotyping system automates the entire karyotyping process from its one click autocapture capabilities to its comprehensive chromosome analysis package.

The PowerGene^™ MacKtype is based upon an Apple® Macintosh® Platform and offers the following key features:

-Trainable classifiers for G, R, and Q banded preparations
-Streamlined automatic image capture and enhancement
-Automatic chromosome separation
-Flexible karyotype format for human and non-human preparations
-Comprehensive ideogram package
-Unique karyotype review function
-Reduced case turnaround time and increased laboratory productivity

The PowerGene^™ MacKtype workstations are available in the following configurations:

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Applied Spectral Imaging, Inc.

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G-, R-, Q- or DAPI-banding with BandView combines high-quality image processing with a complete working environment for the cytogeneticist.

BandView brings you:

• Powerful automated karyotyping tools
• Superb image resolution
• Advanced enhancement algorithms
• Convenient image gallery to scan metaphases and karyotypes
• Multi-language database Case Data Manager (CDM) with link and reports
• Access to patient data statistics according to the parameters you choose
• Seamless upgrade to SKY™ and FISHView™ (no need to change the camera, database or microscope)

Features and Benefits

• Karyotype brightfield and fluorescent samples
• Acquire images
• Enhance images
• Generate reports
• Manage cases
• Installation and support

Karyotype Brightfield and Fluorescent Samples

• No loss of original image data during analysis
• Automated classification of brightfield or fluorescently stained metaphases
• Proprietary background subtraction enables automatic chromosome segmentation on sub-optimal metaphases
• Easy separation of touching and overlapping chromosomes
• Chromosome straightening without losing original image data
• 400-, 550-, 850-band level ideograms with instant access to ISCN nomenclature and band size
• Ideogram editor to display breakpoints and translocations in the ideogram
• Robust same-case comparison tool to compare chromosomes in the same pair
• Cross-application comparison tool to compare band, FISH and SKY™ analysis
• Dynamic karyotype table to fit any number of chromosomes

Acquire Images

• High resolution images with 12-bit digital CCD-camera (1280x1024 pixels)
• Live-view image display throughout image acquisition
• Extremely low dark noise allowing for long exposure times
• Automatic exposure for brightfield, phase contrast and epi-fluorescence
• Easy graphic user interface for quick image acquisition
• Convenient image gallery to preview images before analysis
• Unlimited joining of frames to create one image from multiple images of a sparse metaphase

Enhance Images

• Automatic background correction which accurately resolves non-uniform microscope illumination to allow optimized segmentation
• Save and apply customized settings, such as the Band Enhancement level, to each new image
• 10-level enhancement of fine band detail in brightfield and fluorescence
• Advanced color control for precise brightness and contrast adjustments
• Restore original contrast and brightness in one click

Generate Reports

• Flexible report generation to include any combination of data, including metaphase with case data reports, physician reports, analyzed cells report and statistics summary report
• On-the-fly generation of case statistics reports according to the parameters you set
• Compatibility with any printer with a Windows XP driver
• Multi-language capabilities for the user interface and reports

Manage Cases

• Powerful search utility that can filter by any field, including text strings
• Multi-language capabilities for the user interface and reports
• Archiving to any storage media
• Convenient image gallery to spot cases by metaphase or karyotype
• Immediate access to all patient data in one mouse click
• Network support for multiple systems and workstations

Installation and Support

• Detailed user manuals and extensive on-line help.
• Free phone support for a year from system installation.
• One-year warranty for the system and a three-year warranty for the computer.

More About Applied Spectral Imaging, Inc.

ASI offers a complete line of cytogenetics imaging products called CytoLabView , which includes its FDA-approved BandView EXPO™ automated karyotyping system, its newly-released FISHView EXPO ™ FISH imaging system and SkyView, the gold standard in multicolor karyotyping. All workstations include a unique manual counting utility and the Case Data Manager (CDM), a powerful and sophisticated patient database. In addition, we offer software packages such as CGHView , as well as perform a cost-effective multicolor upgrade to SKYView EXPO™ for the most complete clinical cytogenetics imaging package available.

ASI developed and commercialized the gold standard in multicolor karyotyping (SKYView EXPO™). SKYView EXPO™ simultaneously identifies and displays all chromosomes in unique colors. Its greatest advantage is that it can immediately point out chromosomal aberrations, which are usually an indication of cancer, birth defects or other serious genetic problems, without the need to know the underlying specific disease.

BandView EXPO™, with 510(k) clearance from the FDA, is aimed at the prenatal and oncology clinical markets. FISHView EXPO™ is a general analysis program geared to all fields that use fluorescent imaging, including cytogenetics, pathology, hematology and many others.

ASI also markets SpectraView™, a general analytical imaging system that analyzes and quantifies information that might otherwise be missed. This accurate quantification means that the physical and chemical properties of a sample can be quickly and categorically identified, characterized or verified. There are potential applications for SpectraView™ in drug discovery, genomics, proteomics, cell biology, and pathology.

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